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prism cycling conditions  (Thermo Fisher)


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    Structured Review

    Thermo Fisher prism cycling conditions
    Prism Cycling Conditions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prism+cycling+conditions/pmc11134313-67-17-17
    Average 90 stars, based on 1 article reviews
    prism cycling conditions - by Bioz Stars, 2026-09
    90/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Juggling cadmium detoxification and zinc homeostasis: A division of labour between the two C. elegans metallothioneins
    Article Snippet: For each qPCR reaction a master-mix was prepared containing 5 μL ROX-Buffer (Roche), 0.1 μL probe, 1 μL primers (10 pM) and made up with H 2 O to 8.8 μL total volume. .. The cDNA template was diluted ten-fold and 2 μL was added. qPCR was performed using the standard ABI Prism cycling conditions (2 min at 50 °C, 10 min at 95 °C, 40 cycles, 15 sec 95°C and 1 min 60 °C). .. Data were analysed via the ABI 7500 Fast Software v2.3 and expression fold change determined by the 2 ˗ΔΔCt method.



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